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A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. reasons could be
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human gene may have intron which bacteria cannot process
human gene may have intron which bacteria cannot process
The process of making recombinant DNA molecule involves the introduction of a desired gene into the DNA of a host that will produce the desired protein.
It becomes difficult sometime to induce a cloned eukaryotic gene to function in a prokaryotic host. In eukaryotic genes the presence of long non-coding introns may prevent correct expression of these genes in prokaryotes, which lack RNA-splicing machinery.
It becomes difficult sometime to induce a cloned eukaryotic gene to function in a prokaryotic host. In eukaryotic genes the presence of long non-coding introns may prevent correct expression of these genes in prokaryotes, which lack RNA-splicing machinery.
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