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Question: Answered & Verified by Expert
A foreign gene is inserted in pBR322 using the restriction endonuclease EcoRI. Which of these is true about the E. coli having this pBR322?
BiologyBiotechnology - Principles and ProcessesNEET
Options:
  • A It will be resistant to both antibiotic ampicillin as well as antibiotic tetracycline
  • B It will be resistant to antibiotic ampicillin but not resistant to antibiotic tetracycline
  • C It will be resistant to antibiotic tetracycline but not resistant to antibiotic ampicillin
  • D It will be neither be resistant to antibiotic tetracycline nor to antibiotic ampicillin
Solution:
1464 Upvotes Verified Answer
The correct answer is: It will be resistant to both antibiotic ampicillin as well as antibiotic tetracycline
The restriction site for EcoRI in pBR322 is not present within the ampicillin-resistant gene or within the tetracycline-resistant gene. Thus, when the foreign DNA is added, there will not be insertional activation of any of these genes and the bacterium will be resistant to both antibiotics.

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