A plasmid has accepted the gene of interest, such as gene coding for streptomycin. How can we make clones of this plasmid?

Question

A plasmid has accepted the gene of interest, such as gene coding for streptomycin. How can we make clones of this plasmid?, Inserting it into a virus to generate multiple copies, Treating it with a restriction enzyme in order to cut the molecule into small pieces, Inserting it into a suitable bacterium in order to produce multiple copies, Running it on a gel electrophoresis in order to determine the size of the gene of interest

MARKS App · Accepted Answer

Plasmid is an extra-chromosomal DNA found in bacteria which can self-replicate. To make multiple copies of a plasmid (a small circular DNA), it should be inserted into a bacterium. A plasmid would not replicate if it were inserted into a virus, as viruses do not contain plasmid and don't have the necessary conditions for its replication. Treating the plasmid with a restriction enzyme will cut into smaller fragments, rendering the plasmid useless. Running the plasmid on a gel (using gel electrophoresis) would only tell us the size of the plasmid and not make clones or copies.

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