A sample was given to the lab technician for figuring out whether the cells of the sample come under the category of prokaryote or eukaryote. Four of the dyes are described in the table below are available to the lab tech for the determining process. Dye Test Acridine orange stains DNA and RNA Osmium tetroxide stains lipids Eosin stains cell cytoplasm Nile blue stains cell nuclei Predict the stain that was used by the lab technician that help her identify whether sample cells will come under the group of prokaryote or eukaryote.

Question

A sample was given to the lab technician for figuring out whether the cells of the sample come under the category of prokaryote or eukaryote. Four of the dyes are described in the table below are available to the lab tech for the determining process. Dye Test Acridine orange stains DNA and RNA Osmium tetroxide stains lipids Eosin stains cell cytoplasm Nile blue stains cell nuclei Predict the stain that was used by the lab technician that help her identify whether sample cells will come under the group of prokaryote or eukaryote., Acridine orange, Eosin, Nile blue, Osmium tetroxide

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The eukaryotic cell has a defined nucleus where it is absent in the prokaryotic cells. Thus, best would be for the lab tech to use the Nile blue dye which will stain the nuclei. Since nuclei are absent in prokaryote, there will no successful staining whereas the eukaryote will get stained and help determine the type of cell sample. DNA, lipids, and cytoplasm is commonly found in both the cell types.

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Dye	Test
Acridine orange	stains DNA and RNA
Osmium tetroxide	stains lipids
Eosin	stains cell cytoplasm
Nile blue	stains cell nuclei