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A student wishes to study the cell structure under a light microscope having $10 \mathrm{X}$ eyepiece and $45 \mathrm{X}$ objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?
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Verified Answer
The correct answer is:
blue
In order to increase the resolution, the specimen must be viewed using either shorter wavelength $(\lambda)$ light or through an imaging medium with a relatively high refractive index or with optical components which have a high numerical aperture (NA). thus, resolution of microscope in inversely proportional to the wavelength of the light used.
$$
\Delta d=\frac{\lambda}{2 n \sin \theta}
$$
Where,
$$
\begin{aligned}
& \lambda=\text { wavelength } \\
& n=\text { refractive index } \\
& \theta=\text { half angle subtended } \\
& \Delta d=\text { shortest distance. }
\end{aligned}
$$
$$
\Delta d=\frac{\lambda}{2 n \sin \theta}
$$
Where,
$$
\begin{aligned}
& \lambda=\text { wavelength } \\
& n=\text { refractive index } \\
& \theta=\text { half angle subtended } \\
& \Delta d=\text { shortest distance. }
\end{aligned}
$$
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