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A student wishes to study the cell structure under a light microscope having 10X eyepiece and 45 X objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?
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The correct answer is:
Blue
Resolving power or resolution is the ability of the lens to distinguish fine details and structure. Specifically it refers to the ability of the lenses to distinguish between two points at a specified distance apart. Resolving power depends on two factors :
(i) Wavelength of light used for illumination
(ii) Power of objective lenses
Resolving power $=\frac{\text { Wavelength of light }}{2 \times \mathrm{NA}}$
Since the limit of resolving power of a microscope is fixed by the structure of light, the shortest wavelength of visible light will give the maximum resolution. Among yellow, green, red and blue light colour. Blue (500 nm) have shortest wavelength so, it will give best resolution.
(i) Wavelength of light used for illumination
(ii) Power of objective lenses
Resolving power $=\frac{\text { Wavelength of light }}{2 \times \mathrm{NA}}$
Since the limit of resolving power of a microscope is fixed by the structure of light, the shortest wavelength of visible light will give the maximum resolution. Among yellow, green, red and blue light colour. Blue (500 nm) have shortest wavelength so, it will give best resolution.
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