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A virus with ssDNA is discovered. Its DNA is amplified by running 10 cycles of PCR. How many dsDNA strands will be formed at the end?
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Polymerase chain reaction (PCR) is a process to amplify nucleic acid sequences. In this process, the DNA is replicated multiples times by adding synthesized primer which attach to the denatured DNA strand and are extended using a thermostable polymerase enzyme -Taq polymerase obtained from a thermophilic bacterium, Thermus aquaticus. DNA is denatured by heating and breaking the H-bonds.
In each cycle of PCR the dsDNA is doubled, so after n cycles the copies of DNA will be .
Now the given sample of viral DNA is single stranded, therefore, in the first cycle it will form a dsDNA. And in the consecutive cycles the dsDNA will double in copies. Therefore, after 10 cycles the number of copies will be .
In each cycle of PCR the dsDNA is doubled, so after n cycles the copies of DNA will be .
Now the given sample of viral DNA is single stranded, therefore, in the first cycle it will form a dsDNA. And in the consecutive cycles the dsDNA will double in copies. Therefore, after 10 cycles the number of copies will be .
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