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An electric current is passed through an aqueous solution of a mixture of alanine (isoelectric point 6.0) glutamic acid (3.2) and arginine (10.7) buffered at \(\mathrm{pH}\) 6. What is the fate of the three acids?
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Glutamic acid migrates to anode at \(\mathrm{pH}\) 6. Arginine is present as a cation and migrates to the cathode. Alanine in a dipolar ion remains uniformly distributed in solution.
Hints : At \(\mathrm{pH}=6\), glutamic acid exists as a dianionic species & migrates to anode while arginine exists as cationic species & moves to cathode. Alanine does not migrate to any electrode at its isoelectric point .
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