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Arrange the steps of PCR in decreasing order of temperature.
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Annealing < Polymerisation < Denaturation
PCR is Polymerase chain reaction. This technique is used to form numerous copies of a specific segment of DNA. Thus large quantities of DNA are obtained that are required for various experiments in medicine, molecular biology, forensic biology etc. The steps are:
Denaturation of the template into single strands. The temperature is 94 degree celsius.
Annealing of primers to each original strand for new strand synthesis. the temperature is 50-60 degree celsius
Polymerisation :Extension of new DNA strands from the primers. The temperature is 72 degree celsius.
Denaturation of the template into single strands. The temperature is 94 degree celsius.
Annealing of primers to each original strand for new strand synthesis. the temperature is 50-60 degree celsius
Polymerisation :Extension of new DNA strands from the primers. The temperature is 72 degree celsius.
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