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Question: Answered & Verified by Expert
Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesised by myeloma cells.
Reason: The nutrient deficient medium allows selection of hybridoma cells.
BiologyStrategies for Enhancement in Food ProductionAIIMSAIIMS 2018 (26 May)
Options:
  • A If both assertion and reason are true and reason is the correct explanation of assertion.
  • B If both assertion and reason are true but reason is not the correct explanation of assertion.
  • C If assertion is true but reason is false.
  • D If both assertion and reason are false.
Solution:
1165 Upvotes Verified Answer
The correct answer is: If both assertion and reason are true and reason is the correct explanation of assertion.
The hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesised by myeloma cells so that myeloma cells could be removed or hybridoma cells could be purified. The unfused myeloma cells die while hybridoma cells survive. The hybridoma cells are allowed to multiply separately.

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