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Question: Answered & Verified by Expert
Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by myeloma cells.
Reason : This medium allows selection of hybridoma cells.
BiologyBiotechnology - Principles and ProcessesAIIMSAIIMS 2008
Options:
  • A If both assertion and reason are true and reason is the correct explanation of assertion
  • B If both assertion and reason are true but reason is not the correct explanation of assertion
  • C If assertion is true but reason is false
  • D If both assertion and reason are false.
Solution:
2602 Upvotes Verified Answer
The correct answer is: If both assertion and reason are true and reason is the correct explanation of assertion
The hybridoma cells are shifted to a medium
deficient in nutrient which cannot be synthesized by myeloma cells so that myleoma cells could be removed or hybridoma cells could be purified. The unfused myleoma cells die while hybridoma cells survive. The hybridoma cells are allowed to multiply separately.

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