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Question: Answered & Verified by Expert
Biolistics (gene-gun) is suitable for
BiologyBiotechnology - Principles and ProcessesNEETNEET 2012 (Mains)
Options:
  • A disarming pathogen vectors
  • B transformation of plant cells
  • C constructing recombinant DNA by joining with vectors
  • D DNA fingerprinting
Solution:
1783 Upvotes Verified Answer
The correct answer is: constructing recombinant DNA by joining with vectors
In biolistic or gene gun method 1-2 $\mu \mathrm{g}$ tungeston or gold particle (called microprojectile) coated with DNA to be used for transformation are accelerated to velocities which enable their entry into plant cells/nuclei. This technique has general applicability to plant species and can be used to deliver DNA into virtually all tissues.

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