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In PCR, each cycle has three steps that are shown in the figures these are
A. Denaturation of DNA Sample-Unwinding of two DNA strand by heating the sample at $92-94^{\circ} \mathrm{C}$.
B. Primer Annealing - Primers as per base pairing rules, get positioned on the exposed nucleotides.
C. Extension of Primers - DNA polymerase recognises primers as 'start' tags and begins to extend the primers using the free nucleotides provided in the reaction and the genomic DNA as template. The DNA doubles with each round of reactions.
A. Denaturation of DNA Sample-Unwinding of two DNA strand by heating the sample at $92-94^{\circ} \mathrm{C}$.
B. Primer Annealing - Primers as per base pairing rules, get positioned on the exposed nucleotides.
C. Extension of Primers - DNA polymerase recognises primers as 'start' tags and begins to extend the primers using the free nucleotides provided in the reaction and the genomic DNA as template. The DNA doubles with each round of reactions.
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