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If alien DNA is introduced using Pst I in pBR322 then transformant will grow on:
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Tetracycline
TetR gene in pBR322 can be cleaved by Bam I and Sal I, while AmpR gene in pBR322 is cleaved by Pst I and Pvu I. If foreign DNA segment is inserted in Pst I site than ampicillin-resistant gene becomes inactive due to insertional inactivation and transformant can be grown on tetracycline containing medium.
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