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If the gene of interest is introduced in a tetracycline-resistant gene region of pBR322, then:
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The host will loose resistance for antibiotic tetracycline.
The recombinant plasmid will loose tetracycline nature due to insertion of foreign DNA and this is known as insertional inactivation but can still be selected out from non recombinants by plating the transformants on tetracycline medium.
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