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If the sequence of bases in the coding strand of a double stranded DNA is 5'-GTTCGAGTC-3', the sequence of bases in its transcript will be
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Verified Answer
The correct answer is:
$5^{\prime}$-GUUCGAGUC-3'
During transcription, one of the two DNA strands functions as template strand and the other functions as coding strand. Template strand serves as template for transcription whereas coding strand does not take part in transcription. Hence, the $m \mathrm{RNA}$ produced has base sequence complementary to template strand while similar to coding strand except that thymine $(\mathrm{T})$ is replaced by uracil (U).
Here,
Coding strand $\rightarrow 5^{\prime}$ GTTCGAGTC $3^{\prime}$
Template strand $\rightarrow 5^{\prime}$ CAAGCTCAG $3^{\prime}$
Transcript $\quad \rightarrow 5^{\prime}$ GUUCGAGUC $3^{\prime}$
Here,
Coding strand $\rightarrow 5^{\prime}$ GTTCGAGTC $3^{\prime}$
Template strand $\rightarrow 5^{\prime}$ CAAGCTCAG $3^{\prime}$
Transcript $\quad \rightarrow 5^{\prime}$ GUUCGAGUC $3^{\prime}$
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