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In E. coil the lac operon gets switched on when
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lactose is present and it binds to the repressor
lactose is present and it binds to the repressor
In the presence of lactose repressor
Jacob and Monod model of an inducible operon
(i) Lactose acts as an inducer that binds to the repressor and forms an inactive repressor.
(ii) The repressor fails to bind to the operator and transcript lac mRNA
(iii) The RNA polymerase binds to the operator and transcript lac mRNA.
(iv) lacmRNA is polycistronic, i.e, produces all three enzymes,
$\beta$-galactosidase, permease and transacetylase.
(v) The lac operon is switched on
In the absence of lactose
(i) When lactose is absent, i gene regulates and produces repressor mRNA which translate repression.
(ii) The repressor protein binds to the operator region of the operon and as a result prevents RNA polymerase to bind to the operon.
(iii) The operon is switched off.
Jacob and Monod model of an inducible operon
(i) Lactose acts as an inducer that binds to the repressor and forms an inactive repressor.
(ii) The repressor fails to bind to the operator and transcript lac mRNA
(iii) The RNA polymerase binds to the operator and transcript lac mRNA.
(iv) lacmRNA is polycistronic, i.e, produces all three enzymes,
$\beta$-galactosidase, permease and transacetylase.
(v) The lac operon is switched on
In the absence of lactose
(i) When lactose is absent, i gene regulates and produces repressor mRNA which translate repression.
(ii) The repressor protein binds to the operator region of the operon and as a result prevents RNA polymerase to bind to the operon.
(iii) The operon is switched off.
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