In the process of transformation, divalent ions are used to make the host competent. The function of such ions is to

Question

In the process of transformation, divalent ions are used to make the host competent. The function of such ions is to, increase the efficiency of DNA entering the bacterium, make the DNA hydrophobic so that it enters the bacterium, force the DNA inside the bacterium by applying heat, break the DNA into smaller fragments for better uptake into the bacterium

MARKS App · Accepted Answer

In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.

Search any question & find its solution

Looking for more such questions to practice?