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Mark the incorrect differences between eukaryotic and prokaryotic transcription.
I. In eukaryotes, the genes are split having exons and introns while in prokaryotes it isn't.
II. The structural gene which is to be transcribed is generally mono-cistronic in prokaryotes and poly-cistronic in eukaryotes.
III. The RNA formed with the help of RNA polymerase in eukaryotes requires further processing to function as m-RNA while in prokaryotes it is directly used as m-RNA.
IV. In eukaryotes, RNA polymerase binds to the promoter region while in prokaryotes it binds to the operator region.
Options:
I. In eukaryotes, the genes are split having exons and introns while in prokaryotes it isn't.
II. The structural gene which is to be transcribed is generally mono-cistronic in prokaryotes and poly-cistronic in eukaryotes.
III. The RNA formed with the help of RNA polymerase in eukaryotes requires further processing to function as m-RNA while in prokaryotes it is directly used as m-RNA.
IV. In eukaryotes, RNA polymerase binds to the promoter region while in prokaryotes it binds to the operator region.
Solution:
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Verified Answer
The correct answer is:
II and IV
In eukaryotes, the genes are split having exons (coding regions) and introns (non-coding regions) while in prokaryotes non-coding regions within coding regions do not occur. The structural gene which is to be transcribed is monocistronic in eukaryotes and polycistronic in prokaryotes. The RNA formed with the help of RNA polymerase in eukaryotes requires further processing (splicing, capping, and tailing) to function as m-RNA while in prokaryotes it is directly used as m-RNA. In both prokaryotes and eukaryotes, RNA polymerase binds to the promoter region
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