Detergents represent the largest industrial application of enzymes amounting to $25-30 \%$ of total sales of enzyme. The enzymes used in detergents must be cost effective, safe to use, and be able to perform the task in the presence of anionic and non-ionic detergents, soaps, oxidants etc, at $\mathrm{pH}$ between $8-10.5 \%$. The chief enzymes used are proteases, $\alpha$-amylase and sometimes cellulase.