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Question: Answered & Verified by Expert
Select the incorrect statement.
BiologyBiotechnology - Principles and ProcessesNEET
Options:
  • A More than 900 restriction enzymes have been isolated from over 230 strains of bacteria.
  • B In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated.
  • C Some key tools for recombinant DNA technology are restriction enzyme, polymerase enzyme, ligase, vectors and host root organisms.
  • D EcoRI cut the DNA between bases A and T only when the sequence GAATTC is present in the DNA.
Solution:
2044 Upvotes Verified Answer
The correct answer is: EcoRI cut the DNA between bases A and T only when the sequence GAATTC is present in the DNA.
EcoRI identifies specific palindromic DNA sequence GAATTC and cuts are specific location away from center between G and A making staggered or sticky end cuts. It breaks phosphodiester bonds and hydrogen bonds.

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