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Question: Answered & Verified by Expert
Select the incorrect statement.
BiologyBiotechnology - Principles and ProcessesNEET
Options:
  • A Insertional inactivation technique helps in identification of the recombinant transformants with non-transformants by forming colourless colonies. 
  • B Insertional inactivation of β- galactosidase leads to the formation of colourless colonies.
  • C Insertional inactivation of β- galactosidase leads to the formation of blue color colonies.
  • D In insertional inactivation, the rDNA is inserted within the coding sequence of an enzyme β- galactosidase.
Solution:
2684 Upvotes Verified Answer
The correct answer is: Insertional inactivation of β- galactosidase leads to the formation of blue color colonies.
Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure. Therefore, alternative selectable markers have been developed, which differentiate recombinants from non-recombinants on the basis of their ability to produce colour. A recombinant DNA is inserted within the coding sequence of enzyme β-galactosidase. This results in insertational inactivation, and the colonies do not produces any colour.

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