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Insertional inactivation of galactosidase leads to the formation of blue color colonies.
Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure. Therefore, alternative selectable markers have been developed, which differentiate recombinants from non-recombinants on the basis of their ability to produce colour. A recombinant is inserted within the coding sequence of enzyme galactosidase. This results in insertational inactivation, and the colonies do not produces any colour.
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