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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet ${ }^R$ ) gene in the pBR322 cloning vector.
Options:
Solution:
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Verified Answer
The correct answer is:
Bam HI and Sal I
The correct answer is option (1), because the restriction sites for restriction endonucleases Bam HI and Sal I are present within the tet ${ }^R$ gene in the pBR322 cloning vector.
Options (2), (3) and (4) are incorrect because the restriction sites of $P$ st I and $P v u I$ are present within the $a m p^R$ gene in the pBR322 cloning vector.
Options (2), (3) and (4) are incorrect because the restriction sites of $P$ st I and $P v u I$ are present within the $a m p^R$ gene in the pBR322 cloning vector.
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