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Question: Answered & Verified by Expert
The figure below is the diagrammatic representation of the $E$. coli vector pBR 322 . Which one of the given options correctly identifies its certain components (s)?

BiologyBiotechnology - Principles and ProcessesNEETNEET 2012 (Screening)
Options:
  • A Ori-original restriction enzyme
  • B rop-reduced osmotic pressure
  • C Hind III, Eco RI-selectable markers
  • D $a m p^{\mathrm{R}}$, tet $t^{\mathrm{R}}-$ antibiotic resistance genes
Solution:
2270 Upvotes Verified Answer
The correct answer is: $a m p^{\mathrm{R}}$, tet $t^{\mathrm{R}}-$ antibiotic resistance genes
Ori represents the site of origin of replication, rop represents the proteins that take part in the replication of plasmid. Hind III, Eco RI are the recognition sites of restriction endonucleases. $a m p^{\mathrm{R}}$ and $t e t^{\mathrm{R}}$ are the antibiotic resistant gene parts.

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