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The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ and ' $Y$ genes:

Options:

Solution:
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Verified Answer
The correct answer is:
The gene ' $X$ ' is responsible for controlling the copy number of the linked DNA and ' $Y$ ' for protein involved in the replication of Plasmid.
Correct answer is option (1), because
' $X$ ' in the given diagram is ori while ' $Y$ ' is rop.
' $X$ ' which is ori is responsible for controlling the copy number of the linked DNA and ' $Y$ which is rop codes for protein involved in the replication of plasmid.
Options (2), (3) and (4) are incorrect as ' $X$ ' and ' $Y$ ' are not related to these functions.
' $X$ ' in the given diagram is ori while ' $Y$ ' is rop.
' $X$ ' which is ori is responsible for controlling the copy number of the linked DNA and ' $Y$ which is rop codes for protein involved in the replication of plasmid.
Options (2), (3) and (4) are incorrect as ' $X$ ' and ' $Y$ ' are not related to these functions.
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