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The packaging of chromatin at a higher level requires an additional set of proteins that are collectively referred to as
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Non histone chromosomal proteins
The nucleosome in chromatin are seen as 'beads on string' structure when viewed under electron microscope. It is further packaged to form Chromatin fibres that are further coiled and condensed at metaphase stage of cell division to form chromosome, this packaging at higher level requires an additional set of proteins collectively referred as NHC protein.
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