The value of numerical aperture of the objective lens of a microscope is 1.25 . If light of wavelength 5000 Å is used, the minimum separation between two points, to be seen as distinct, will be:

Question

The value of numerical aperture of the objective lens of a microscope is 1.25 . If light of wavelength 5000 Å is used, the minimum separation between two points, to be seen as distinct, will be:, 0.48 μ m, 0.24 μ m, 0.38 μ m, 0.12 μ m

MARKS App · Accepted Answer

R e s o l v i n g l i m i t = 0.61 λ d = 0.61 × 5000 × 10 - 10 1.25 = 0.24 μ m

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