In thin layer chromatography using hexane, the least polar compound will rise to maximum height and most polar compound will rise to minimum height.

The retardation factor of each spot can be determined by dividing the distance that a particular compound has travelled by the distance between solvent front and initial spotting site. This parameter is dependent on the thin layer chromatographic plate and the solvent.

$\therefore {\mathrm{R}}_{\mathrm{f}}$ value for most polar compound

$=\frac{2}{8}=0.25$

$=25\times {10}^{-2}$