During denaturation, \(2^{\circ}\) and \(3^{\circ}\) structures of proteins are destroyed but \(1^{\circ}\) structure remains intact. As a result of denaturation, the globular proteins (soluble in \(\mathrm{H}_2 \mathrm{O}\) ) are converted into fibrous proteins (insoluble in \(\mathrm{H}_2 \mathrm{O}\) ) and their biological activity is lost. For example, boiled egg which contains coagulated proteins cannot be hatched.