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Question: Answered & Verified by Expert
Which one is a true statement regarding DNA polymerase used in PCR?
BiologyBiotechnology - Principles and ProcessesNEETNEET 2012 (Screening)
Options:
  • A It is used to ligate introduced DNA in recipient cells
  • B It serves as a selectable marker
  • C It is isolated from a virus
  • D It remains active at high temperature
Solution:
2349 Upvotes Verified Answer
The correct answer is: It remains active at high temperature
Polymerase Chain Reaction (PCR) is used to amplify a DNA segment or to synthesize in vitro the multiple copies of gene (or DNA) of interest, using two sets of primers and the enzyme DNA polymerase. This enzyme is isolated from a bacterium Thermus aquaticus and it remains active during the high temperature but high temperature induced denaturation of double stranded DNA.

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