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Question: Answered & Verified by Expert
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
BiologyBiotechnology - Principles and ProcessesNEETNEET 2010 (Screening)
Options:
  • A $5^{\prime}-\mathrm{CGTTCG}-3^{\prime}$
    3'-ATGGTA-5'
  • B 5'-GATATG-3' 3'-CTACTA-5
  • C $5^{\prime}-\mathrm{GAATTC}-3^{\prime}$ $3^{\prime}-\mathrm{CTTAAG}-5^{\prime}$
  • D 5'-CACGTA-3' $3^{\prime}-\mathrm{CTCAGT}-5^{\prime}$
Solution:
2908 Upvotes Verified Answer
The correct answer is: $5^{\prime}-\mathrm{GAATTC}-3^{\prime}$ $3^{\prime}-\mathrm{CTTAAG}-5^{\prime}$
The palindromic base sequence is a sequence in double-stranded nucleic acids that reads the same on both strands when reading one strand from left to right and the other from right to left (ie, both strands are read $5^{\prime} \rightarrow 3$ ). Out of the given options
$$
\begin{aligned}
& 5^{\prime}-\text { GAATTC-3 } \\
& 3 \text {-CTTAAG-5 }
\end{aligned}
$$
is a palindromic sequence that can be cut at about the middle by particular restriction enzyme.

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