Analyze the statements given below regarding the thermal cycler and select the option that shows incorrect statements. I. Annealing is also called the hybridization of oligonucleotide (primer). II. During extension, D N A polymerase polymerizes nucleotides in 5 ′ → 3 ′ direction. III. Denaturation is a process of formation of H-bonds.

Question

Analyze the statements given below regarding the thermal cycler and select the option that shows incorrect statements. I. Annealing is also called the hybridization of oligonucleotide (primer). II. During extension, D N A polymerase polymerizes nucleotides in 5 ′ → 3 ′ direction. III. Denaturation is a process of formation of H-bonds., I a n d I I I, O n l y I I, O n l y I I I, I a n d I I

MARKS App · Accepted Answer

The pairing of denatured D N A upon cooling is known as annealing. In PCR, the synthesis of nucleotide chain proceeds in 5 ′ → 3 ′ direction. PCR stands for polymerase chain reaction. In this reaction, multiple copies of the gene (or DNA) of interest are amplified.

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