Plasmid pBR 322 has PstI restriction enzyme site within gene amp R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactoside production and the recombinant plasmid is inserted in an E. coli strain

Question

Plasmid pBR 322 has PstI restriction enzyme site within gene amp R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactoside production and the recombinant plasmid is inserted in an E. coli strain, it will not be able to confer ampicillin resistance to the host cell., the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside., it will lead to lysis of host cell., it will be able to produce a novel protein with dual ability.

MARKS App · Accepted Answer

pBR 322 has two antibiotic-resistant genes for ampicillin (ampR) and tetracycline (tetR). Due to the insertion of the gene in the ampR region, insertional inactivation results in the inactivation of the ampicillin gene and hence the organisms become sensitive to this antibiotic and do not grow in the media containing this antibiotic.

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