Join the Most Relevant JEE Main 2025 Test Series & get 99+ percentile! Join Now
Search any question & find its solution
Question: Answered & Verified by Expert
Primers used in PCR must be:
BiologyBiotechnology and Its ApplicationsNEET
Options:
  • A 3'-end specific
  • B 5'-end specific
  • C It can be 3'-end specific or 5'-end specific.
  • D Primers are not needed in PCR.
Solution:
2836 Upvotes Verified Answer
The correct answer is: 3'-end specific
Since the replication of DNA occurs in a 5' to 3' direction, the primers used should be complementary to 3' end of template DNA. The primers used in PCR are the small primers of DNA.

Looking for more such questions to practice?

Download the MARKS App - The ultimate prep app for IIT JEE & NEET with chapter-wise PYQs, revision notes, formula sheets, custom tests & much more.