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The function of leghaemoglobin during biological nitrogen fixation in root nodules of legumes is to
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protect nitrogenase from oxygen.
Rhizobium is the nitrogen fixing bacteria present in root nodules of leguminous plants. Roots of a legume secrete chemical attractants flavonoids and betaines. Bacteria collect over the root hairs, release nod factors that causes curling of root hairs around the bacteria, degradations of cell wall and formation of an infection thread enclosing bacteria. The infected cortical cells differentiate and start dividing. It produces swelling of nodules and the infected cells enlarge. Bacteria stop dividing and form irregular polyhedral structures called bacteroids. The host cells develops a pinkish coloured pigment called leghaemoglobin. It is an oxygen scavenger and protects nitrogenase from oxygen. Symbiotic nitrogen fixation requires co-operations of ' $\mathrm{Nod}$ ' genes of legumes, 'nod' 'nif and 'fix' genes of bacteria.
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