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Question: Answered & Verified by Expert
The vector for T-DNA is
BiologyBiotechnology - Principles and ProcessesJIPMERJIPMER 2014
Options:
  • A Thermus aquaticus
  • B Salmonella typhimurium
  • C Agrobacterium tumefaciens
  • D Escherichia coli
Solution:
1348 Upvotes Verified Answer
The correct answer is: Agrobacterium tumefaciens
- Plant transformation mediated by the soil pathogen, Agrobacterium tumefaciens has become the most used method for plant transformation.
- It naturally infects the wound sites in dicots causing the crown gall disease. It is capable to transfer T-DNA of the Tumour inducing (Ti) plasmid into the nucleus of infected cells.
- It will then integrate into the host genome and is transcribed, causing the crown gall disease.
- Thus, the correct answer is option C.

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