The vector must also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contain unique recognition sites for several restriction enzymes in a small region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:

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The vector must also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contain unique recognition sites for several restriction enzymes in a small region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:, Ability to separate DNA fragments, Flexibility in the choice of restriction enzyme, Flexibility in selectable marker, Ability of DNA to mutate itself

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Multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites. Multiple cloning site allows considerable flexibility in the choice of restriction enzymes for cloning.

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