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Question: Answered & Verified by Expert
What happens to the tetracycline resistance gene when a foreign DNA is ligated at the BamH I site?
BiologyBiotechnology - Principles and ProcessesNEET
Options:
  • A It remains intact
  • B It gets activated
  • C It gets inactivated
  • D It gets eliminated
Solution:
2084 Upvotes Verified Answer
The correct answer is: It gets inactivated
The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA) The transformants growing on ampicillin containing medium are then transferred on a medium containing tetracycline. The recombinants will grow in ampicillin containing medium but not on that containing tetracycline.

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