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Question: Answered & Verified by Expert
Which one of the following restriction enzyme can cut TetR site of pBR322?
BiologyBiotechnology - Principles and ProcessesNEET
Options:
  • A Pvu I, Pst I
  • B BamH I, Sal I
  • C Pvu II
  • D EcoR I
Solution:
2419 Upvotes Verified Answer
The correct answer is: BamH I, Sal I
pBR 322 plasmid has two antibiotic resistant gene one for Tertracyclin resistant and other for amphicilin resistant, these two functions as selectable marker gene. TetR site has two restriction enzyme recognition site, BamH I and Sal I site.

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